對日本慢生根瘤菌USDA110進(jìn)行Tn5轉(zhuǎn)座子誘變，產(chǎn)生缺陷突變體。篩選根瘤菌中硝酸還原酶活性降低的菌株，通過實(shí)驗(yàn)發(fā)現(xiàn)硝酸還原活性降低后固氮能力與對硝酸鹽的耐性提升。

參考文獻(xiàn)：María Camacho, Araceliburgos R d C ,ManuelA. Chamber-Pérez.Nitrogen fixation in transposon mutants from Bradyrhizobium japonicum USDA 110 impaired in nitrate reductase.[J].Journal of Plant Physiology, 2003, 160(4):377-386.