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pCW26
pCW26
規(guī)格:
價(jià)格:
編號(hào):B161752
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
pCW26
商品貨號(hào)
B161752
Designations
pCW26
GenBank Number

M80261

培養(yǎng)基
Species
Homo sapiens, human
Depositors
B Demple
Vector
Construct size (kb): 4.239999771118164
Insert
DNA: cDNA
Insert lengths(kb): 1.279000043869019
Tissue: placenta
Gene product: apurinic/apyrimidinic (abasic) endonuclease [APE]
Insert Size (kb)
1.279
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 1.2, 0.9; BamHI--4.8; PstI--2.9, 1.9.
Restriction digests of the clone give the following sizes (kb): BglII--4.3; EcoRI--2.9, 1.3; PstI--3.8, 0.5; PvuII--2.5, 1.1, 0.4 (doublet); SacI--3.1, 1.2.
The insert contains the following restriction sites (bp from the 5' end): BglII--310; PstI--852; PvuII--97, 436; SacI--168.
Contains the complete coding sequence, including 122 nt of 5' untranslated region (with at least one translational stop codon in each reading frame), and 204 nt of 3' untranslated region.
The GenBank accession number for the genomic sequence is M99703. This record contains correct information for 4 sites in the cDNA sequence (2 in coding regions, 2 in non-coding regions) that were reported in error.
Transcription from the T3 promoter gives the sense strand.
References

Demple B, et al. Cloning and expression of APE, the cDNA encoding the major human apurinic endonuclease: definition of a family of DNA repair enzymes. Proc. Natl. Acad. Sci. USA 88: 11450-11454, 1991. PubMed: 1722334

Bruce Demple, personal communication

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