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pCW26
pCW26
規(guī)格:
價格:
編號:B217134
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pCW26
商品貨號
B217134
Designations
pCW26
GenBank Number

M80261

培養(yǎng)基
Species
Homo sapiens, human
Vector
Construct size (kb): 4.239999771118164
Insert
DNA: cDNA
Insert lengths(kb): 1.279000043869019
Tissue: placenta
Gene product: apurinic/apyrimidinic (abasic) endonuclease [APE]
Insert Size (kb)
1.279
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 1.2, 0.9; BamHI--4.8; PstI--2.9, 1.9.
Restriction digests of the clone give the following sizes (kb): BglII--4.3; EcoRI--2.9, 1.3; PstI--3.8, 0.5; PvuII--2.5, 1.1, 0.4 (doublet); SacI--3.1, 1.2.
The insert contains the following restriction sites (bp from the 5' end): BglII--310; PstI--852; PvuII--97, 436; SacI--168.
Contains the complete coding sequence, including 122 nt of 5' untranslated region (with at least one translational stop codon in each reading frame), and 204 nt of 3' untranslated region.
The GenBank accession number for the genomic sequence is M99703. This record contains correct information for 4 sites in the cDNA sequence (2 in coding regions, 2 in non-coding regions) that were reported in error.
Transcription from the T3 promoter gives the sense strand.
References

Demple B, et al. Cloning and expression of APE, the cDNA encoding the major human apurinic endonuclease: definition of a family of DNA repair enzymes. Proc. Natl. Acad. Sci. USA 88: 11450-11454, 1991. PubMed: 1722334

Bruce Demple, personal communication

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