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當前位置: 首頁 > ATCC代理 > Clone 9 [UTR-02817, WI-7764] B161827
Clone 9 [UTR-02817, WI-7764]
Clone 9 [UTR-02817, WI-7764]
規格:
價格:
編號:B161827
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Clone 9 [UTR-02817, WI-7764]
商品貨號
B161827
Designations
Clone 9 [UTR-02817, WI-7764]
GenBank Number

M58603

培養基
Species
Homo sapiens, human
Depositors
C Scheidereit, Max Planck Institut fur Molekulare Genetik
Vector
Name of construct: clone 9
Other name(s): WI-7764; UTR-02817
Excise insert: EcoRI
Size of construct (kb): 6.8
Markers: ampR

Name of vector: pBluescript II SK
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli

Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7, T3
replicon: pMB1, f1
Insert
Genome: Homo sapiens
Gene symbol: NFKB1
Genomic copy number: unique
Gene name: nuclear factor of kappa light chain gene enhancer in B-cells 1 (p105)
Contains complete coding sequence?: Y
Chromosome: 4; Localization: 4 q24; 4, 538.149999 cR
Tissue: BJA-B cell line
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 3.821
Cross references: DNA Seq. Acc.: M58603
Insert Size (kb)
3.821
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products
ATCC 33694
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): PstI--4.0, 1.9, 1.0; BglI--3.0, 2.4, 1.2, 0.3; EcoRI--3.9, 3.0; SalI--6.9.
Restriction digests of the clone give the following sizes (kb): PstI--4.0, 1.9, 1.0; BglI--3.0, 2.4, 1.2, 0.3; EcoRI--3.9, 3.0; SalI--6.9.
The insert includes the following restriction sites (nt from the 5' end): PvuII--958, 1172, 2576, 2739, 2827, 2849, 3572, 3589; XbaI--1900, 2722, 3286; BamHI--448; SmaI--284; HindIII--3563.
Contains the complete coding sequence, plus 5' (397 bp) and 3' (520 bp, including the stop codon) untranslated regions. Extends 196 bp beyond the reported sequence in the 3' direction.
References

Meyer R, et al. Cloning of the DNA-binding subunit of human nuclear factor kappaB: the level of its mRNA is strongly regulated by phorbol ester or tumor necrosis factor alpha. Proc. Natl. Acad. Sci. USA 88: 966-970, 1991. PubMed: 1992489

Claus Scheidereit, personal communication

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