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當前位置: 首頁 > ATCC代理 > pSW 11-1 [pSW11-1] B161877
pSW 11-1 [pSW11-1]
pSW 11-1 [pSW11-1]
規格:
價格:
編號:B161877
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pSW 11-1 [pSW11-1]
商品貨號
B161877
Designations
pSW 11-1 [pSW11-1]
GenBank Number

L00045

培養基
Species
Homo sapiens, human
Depositors
RA Weinberg
Applications
NIH 3T3 cells were transformed with DNA from the colon adenocarcinoma SW480 cell line to produce the cell line, SW2-3. DNA from SW2-3 was used to isolate the clone.
Vector
Construct size (kb): 3.900000095367432
Insert
DNA: cDNA
Insert lengths(kb): 1.100000023841858
Tissue: SW480 cell line DNA transferred into NIH 3T3 cells
Gene product: Kirsten rat sarcoma 2 viral (v-Ki-ras2) oncogene homolog( c-Kirsten-ras proto-oncogene, oncogene KRAS2, cellular c-Ki-ras2 proto-oncogene) [KRAS2]
Insert Size (kb)
1.100
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI--3.8; EcoRI--3.1, 0.6; PstI/EcoRI--2.6, 0.6, 0.5.
Detects a TaqI polymorphism mapping to chromosome 6.
A full-length Ki-ras cDNA capable of transformation if given a promoter. The PstI site on the 3' side of the insert appears to be missing. The insert contains HinfI (3), TaqI (3), Sau96I (2) and EcoRI sites.
NIH 3T3 cells were transformed with DNA from the colon adenocarcinoma SW480 cell line to produce the cell line, SW2-3. DNA from SW2-3 was used to isolate the clone.
References

Siracusa LD, et al. A molecular genetic linkage map of mouse chromosome 2. Genomics 6: 491-504, 1990. PubMed: 1970329

Threadgill DW, et al. Syntenic conservation between humans and cattle: II. human chromosome 12. Genomics 8: 29-34, 1990. PubMed: 2081597

McCoy MS, et al. Human colon carcinoma Ki-ras2 oncogene and its corresponding proto-oncogene. Mol. Cell. Biol. 4: 1577-1582, 1984. PubMed: 6092920

Barbara Weiffenbach, personal communication

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