黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > Transmissible gastroenteritis virus, porcine B162002
Transmissible gastroenteritis virus, porcine
Transmissible gastroenteritis virus, porcine
規(guī)格:
貨期:
編號:B162002
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Transmissible gastroenteritis virus, porcine
商品貨號
B162002
Classification
Coronaviridae, Coronavirus
Agent
Transmissible gastroenteritis virus, porcine
Strain
Miller (TC-adapted)
Common Name
Transmissible gastroenteritis virus, porcine
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
This item is derived by adaptation of virus isolated from small intestine of gnotobiotic infected pig (ATCC VR-743) to ST cells (ATCC CRL-1746) at ATCC.
Product Format
frozen
Storage Conditions
?Store at -70°C or colder
Comments
This item is derived by adaptation of virus isolated from small intestine of gnotobiotic infected pig (ATCC VR-743) to ST cells (ATCC CRL-1746) at ATCC.
Recommended Host
Production Host:ST cells (ATCC CRL-1746)

Recommended Host: ?ST cells (ATCC? CRL-1746?)

Growth Conditions
Plant cells 24 hours in advance and infect when cultures are 90-100% confluent. Remove medium and rinse cells twice with VGM without TPCK-treated trypsin. Leave the second wash over the cells for 3 hours at 37.0°C in a humidified 5% CO2 atmosphere. Remove the second wash from the cells and then inoculate with a small volume of virus diluted with VGM supplemented with TPCK- treated trypsin (1 μg/ mL) to provide a MOI of about 0.1. Adsorb for 1 hour at 37.0°C in a humidified 5% CO2 atmosphere. End adsorption by adding VGM supplemented with 1 μg/ mL of TPCK- treated trypsin.
Incubate infected culture for 3 days at 37.0°C in a humidified 5% CO2 atmosphere, until CPE are well advanced through 70-80% of the culture.
Name of Depositor
LJ Saif
Source
Small intestine of neonatal pig with porcine transmissible gastroenteritis.
References

Bohl EH, et al. Antibody responses in serum, colostrum, and milk of swine after infection or vaccination with transmissible gastroenteritis virus. Infect. Immun. 6: 289-301, 1972. PubMed: 4629259

Bohl EH, Kumagai T. The use of cell cultures for the study of TGE virus of swine. Proc. U.S. Livestock Sanit. Assoc. 69: 343-350, 1965.

  • 王經(jīng)理
    手機(jī)號：17280875617
    QQ：1438578920
  • 胡經(jīng)理
    手機(jī)號：13345964880
    QQ：2438244627
  • 周經(jīng)理
    手機(jī)號：17757487661
    QQ：1296385441
  • 于經(jīng)理
    手機(jī)號：18067160830
    QQ：2088210172
  • 沈經(jīng)理
    手機(jī)號：19548299266
    QQ：2662369050
  • 李經(jīng)理
    手機(jī)號：13626845108
    QQ：972239479