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Storage Conditions

Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section

Preceptrol®

no

Genotype

P2old+ mcrA delta(mcrBC-hsdRMS-mrr)102 supE supF

Type Strain

no

Comments

Not tolerant to sequences that form secondary structure. Permits Spi selection of recombinant phage

A P2 lysogen of Escherichia coli PMC104 (ATCC 47061)

High-efficiency, methylation-tolerant host for bacteriophage lambda with or without amber mutations. Not tolerant to sequences that form secondary structure. Permits Spi selection of recombinant phage.

This strain is being made readily available to the scientific community with the proviso that it not be used to form the basis of a product for commercial sale or be included in any commercial product.

Medium

ATCC^® Medium 1065: LB Agar/Broth, Miller

Growth Conditions

Temperature: 37.0°C

Name of Depositor

JP Doherty

References

Doherty JP, et al. Escherichia coli host strains SURE (TM) and SRB fail to preserve a palindrome cloned in lambda phage: improved alternate host strains. Gene 124: 29-35, 1993. PubMed: 8440479

Judith P Doherty, personal communication