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pMP81 [lambdaMP14]
pMP81 [lambdaMP14]
規格:
貨期:
編號:B162420
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pMP81 [lambdaMP14]
商品貨號
B162420
Designations
pMP81 [lambdaMP14]
GenBank Number

X13897

Species
Homo sapiens, human
Depositors
JS Miles
Vector
Construct size (kb): 4.5
Insert
DNA: cDNA
Insert lengths(kb): 1.759999990463257
Tissue: liver
Gene product: cytochrome P450, subfamily IIA (phenobarbital-inducible)(coumarin 7-hydroxylase) [CYP1]
Alleles: A1, A1, A2, A2, A1, A2
Insert Size (kb)
1.760
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): AccI--4.5; XhoI--uncut; HindIII--4.5; EcoRI--2.8, 1.75; KpnI--4.5.
CYP2A (pMP12) and CYP2B (pMP17) hybridize to the same MluI (350 kb), NotI (520 kb), and SstII (350 kb) fragments, but to different BssHII, NaeI, NarI, SfiI, and SmaI fragments.
This probe detects the SstI (SacI) RFLP originally reported by Wainwright.
Three sizes of transcripts (1.9 kb, 2.7 kb, and 3.8 kb) are identified in Northern blots from human liver, with the 1.9 kb transcript being most prevalent. Each transcript demonstrated significant inter-individual variation (n=6).
Enzyme(s) not detecting polymorphism: ApaI, BamHI, BglII, EcoRI, EcoRV, HaeIII, HindIII, HinfI, MspI, PstI, PvuII, RsaI, TaqI and XmnI.
References

Miles JS, et al. Close linkage of the human cytochrome P450IIA and P450IIB gene subfamilies: implications for the assignment of substrate specificity. Nucleic Acids Res. 17: 2907-2917, 1989. PubMed: 2726448

Wainwright BJ, et al. RFLP for a human cytochrome P-450 gene at 19q13.1-qter (HGM8 provisional designation CYP1). Nucleic Acids Res. 13: 4610, 1985. PubMed: 4011450

John S Miles, personal communication

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