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pMP81 [lambdaMP14]
pMP81 [lambdaMP14]
規(guī)格:
價格:
編號:B242056
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pMP81 [lambdaMP14]
商品貨號
B242056
Designations
pMP81 [lambdaMP14]
GenBank Number

X13897

培養(yǎng)基
Species
Homo sapiens, human
Vector
Construct size (kb): 4.5
Insert
DNA: cDNA
Insert lengths(kb): 1.759999990463257
Tissue: liver
Gene product: cytochrome P450, subfamily IIA (phenobarbital-inducible)(coumarin 7-hydroxylase) [CYP1]
Alleles: A1, A1, A2, A2, A1, A2
Insert Size (kb)
1.760
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): AccI--4.5; XhoI--uncut; HindIII--4.5; EcoRI--2.8, 1.75; KpnI--4.5.
CYP2A (pMP12) and CYP2B (pMP17) hybridize to the same MluI (350 kb), NotI (520 kb), and SstII (350 kb) fragments, but to different BssHII, NaeI, NarI, SfiI, and SmaI fragments.
This probe detects the SstI (SacI) RFLP originally reported by Wainwright.
Three sizes of transcripts (1.9 kb, 2.7 kb, and 3.8 kb) are identified in Northern blots from human liver, with the 1.9 kb transcript being most prevalent. Each transcript demonstrated significant inter-individual variation (n=6).
Enzyme(s) not detecting polymorphism: ApaI, BamHI, BglII, EcoRI, EcoRV, HaeIII, HindIII, HinfI, MspI, PstI, PvuII, RsaI, TaqI and XmnI.
References

Miles JS, et al. Close linkage of the human cytochrome P450IIA and P450IIB gene subfamilies: implications for the assignment of substrate specificity. Nucleic Acids Res. 17: 2907-2917, 1989. PubMed: 2726448

Wainwright BJ, et al. RFLP for a human cytochrome P-450 gene at 19q13.1-qter (HGM8 provisional designation CYP1). Nucleic Acids Res. 13: 4610, 1985. PubMed: 4011450

John S Miles, personal communication

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