Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Porcine reproductive and respiratory syndrome virus strain ATCC VR-2332, complete genome
Virus may cause disease in swine, specifically respiratory disease and stillbirth in pregnant sows. The virus is a fastidious, non-hemagglutinating enveloped RNA virus. It is heat labile and grows best at 34°C to 35°C. Media should be equilibrated in a 5% CO2 incubator overnight before use. Once fed, virus must be kept in sealed flask or container that prevents further exchange of CO2 with culture. Inoculate 80-90% confluent monolayer of cell.
Note: This material is cited in a U.S. and/or other Patent and may not be used to infringe the patent claims.Temperature: 35°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 35°C, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-5 days at 35°C in a humidified atmosphere, until CPE is progressed through 80% of the monolayer.
Chaladek DW, et al. Method of growing and attenuating a viral agent associated with mystery swine disease. US Patent 5,476,778 dated Dec 19 1995
Nucleotide (GenBank) : U87392.3 Porcine reproductive and respiratory syndrome virus strain ATCC VR-2332, complete genome
