Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
The cells are completely dependent on interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for long term growth.
The cells DO NOT RESPOND to interleukin 5 (IL-5). TF-1 cells respond to a variety of other lymphokines and cytokines such as interleukin 1 (IL-1), interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 9 (IL-9),Interleukin 11 (IL-11), interleukin 13 (IL-13), stem cell factor (SCF), leukemia inhibitory factor (LIF) and nerve growth factor (NGF). TF-1 cells do not express glycophorin A or carbonyl anhydrase I.
The morphological and cytochemical features, and the constitutive expression of globin genes, indicate the commitment of the cells to the erythroid lineage. Hemin and delta-aminolevulinic acid induce hemoglobin synthesis, and TPA induces dramatic differentiation of the TF-1 cells into macrophage-like cells.
The TF-1 cell line is unique because of its responsiveness to multiple cytokines.
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