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HCC2157 BL
HCC2157 BL
規格:
貨期:
編號:B162580
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HCC2157 BL
商品貨號
B162580
Organism
Homo sapiens, human
Tissue
peripheral blood
Cell Type
lymphoblast B lymphoblast; immortalized with Epstein-Barr virus (EBV)Epstein-Barr virus (EBV) transformed
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension, multicell aggregates
Biosafety Level
2 [Cells contain Herpes Virus (Epstein-Barr Virus)]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
normal
Age
48 years adult
Gender
female
Ethnicity
Black
Storage Conditions
liquid nitrogen vapor phase
Derivation

HCC2157 BL is a B lymphoblastoid cell line initiated by Epstein-Barr virus (EBV) transformation of peripheral blood lymphocytes obtained from the same patient as HCC2157. 

HCC2157 was initiated from a 48-year-old black female with primary breast ductal carcinoma, and is available as ATCC CRL-2340.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 105 viable cells/mL. Maintain cultures at cell concentrations between 5 x 105 and 2 x 106 viable cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 12
D16S539: 11,12
D5S818: 12,13
D7S820: 10,12
THO1: 8,9
TPOX: 9,11
vWA: 14,18
Name of Depositor
AF Gazdar, AK Virmani
References

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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