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HCC2157
HCC2157
規格:
貨期:
編號:B164571
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HCC2157
商品貨號
B164571
Organism
Homo sapiens, human
Tissue
mammary gland; breast
Cell Type
Epithelial
Product Format
frozen
Morphology
epithelial
Culture Properties
suspension, multicell aggregates, Cells in suspension show an inclination to form duct-like structures and gland-like structures.
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
TNM stage IIIA, grade 2,primary ductal carcinoma
Age
48 years adult
Gender
female
Ethnicity
Black
Applications
HCC2157 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.
The cells are negative for expression of estrogen receptor (ER -), but positive for expression of progesterone receptor (PR +).
The cells are positive for expression of Her2-neu and for expression of p53.
The cells form ducts and morula-like structures (hollow spherical structures lined with a single layer of cells).
Storage Conditions
liquid nitrogen vapor temperature
Karyotype
Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22%
Images
Cell Micrograph of ATCC CRL-2340, HCC2157
Derivation
This cell line was initiated on 3/4/96 and took 8 months to establish.
An EBV transformed lymphoblastoid cell line (HCC2157 BL) from the same patient is available as ATCC CRL-2341.
Clinical Data
Black
female
48 years

Receptor Expression
estrogen receptor, negative
progesterone receptor, positive
Oncogene
her2/neu +, p53 +
Genes Expressed
Epithelial glycoprotein 2 [EGP2]; cytokeratin 19
Cellular Products
Epithelial glycoprotein 2 [EGP2]; cytokeratin 19
Comments
The tumor was classified as TNM stage IIIA, grade 2, ductal carcinoma with 1 out of 9 lymph node metastasis.
The cells form ducts and morula-like structures (hollow spherical structures lined with a single layer of cells).
HCC2157 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.
An EBV transformed lymphoblastoid cell line (HCC2157 BL) from the same patient is available as ATCC CRL-2341.
Complete Growth Medium
RPMI-1640 medium, 95%; fetal bovine serum, 5%
Subculturing
Cultures can be maintained by addition of fresh medium. Cultures grow as floating aggregates of large irregular cell clusters.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 11
D5S818: 13
D7S820: 10,12
THO1: 8,9
TPOX: 9,11
vWA: 14,18
Name of Depositor
AF Gazdar, AK Virmani
Year of Origin
1996
References

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

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