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當(dāng)前位置: 首頁 > ATCC代理 > Murine leukemia virus B162795
Murine leukemia virus
Murine leukemia virus
規(guī)格:
貨期:
編號:B162795
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Murine leukemia virus
商品貨號
B162795
Classification
Retroviridae, Gammaretrovirus, Mammalian virus group
Deposited As
Abelson Murine leukemia virus
Agent
Murine leukemia virus
Strain
P160 (original isolate) (B-tropic)
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
tissue, animal
Bethesda Maryland, United States
Product Format
frozen
Storage Conditions
-70° or colder
Comments
Assays for transformation: 1) NIH 3T3 cells, Nature (Lond.) 253:729, 1975;2) IL-3 factor independence on IL-3 dependent cells, Cell 41: 685,1985;3) In vitro bone marrow (Whitlock-Witte culture), Cell 32: 90,1983.
Assays for transformation: 1) NIH 3T3 cells, Nature (Lond.) 253:729, 1975;2) IL-3 factor independence on IL-3 dependent cells, Cell 41: 685,1985;3) In vitro bone marrow (Whitlock-Witte culture), Cell 32: 90,1983.
Effect on Host
Yes, in vitro effects: frank refractile macroscopic foci on confluent monolayers
In vitro: Frank refractile macroscopic foci on confluent monolayers.? Syncytia formation (plaques) on SC (ATCC CCL-165) cells.??????????? In vitro: Original isolate reported to produce lymphosarcomas in BALC/c mice.
Yes, in vivo effects: induces lymphosarcomas in BALB/c mice
Yes, in vitro effects: syncytium formation (plaques) on XC cells
Recommended Host

Recommended Host:

?Preferred Host: SC-1 (ATCC CRL 1404)

?Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent).

?Recommended Virus Growth Medium:

?Alternate Host(s): NIH 3T3 murine hematopoietic cells, rodent fibroblasts

?Alternate Host(s): NIH 3T3 murine hematopoietic cells, rodent fibroblasts

Host Range: mice, rats

Growth Conditions
Temperature: 37.0°C
Duration: 7-8 days
Cryopreservation
Storage temperature: -70 or lower
Name of Depositor
D Baltimore
Special Collection
ATCC
Source
tissue, animal
Bethesda Maryland, United States
References

Abelson HT, Rabstein LS. Influence of prednisolone on Moloney leukemogenic virus in BALB-c mice. Cancer Res. 30: 2208-2212, 1970. PubMed: 4918190

Abelson HT, Rabstein LS. Lymphosarcoma: virus-induced thymic-independent disease in mice. Cancer Res. 30: 2213-2222, 1970. PubMed: 4318922

The virus was isolated from lymph nodes of a prednisolone-treated BALB/c mouse which had been inoculated with Moloney murine leukemia virus. From the time of isolation, the isolate appears to have contained Moloney murine leukemia virus, as well as the new Abelson virus.

B-tropic

B-tropic

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