Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Mumps virus strain MuV-IA, complete genome
Temperature: 37°C
Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.001-0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: 3-7 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Boddicker JD, et al. Real-time reverse transcription-PCR assay for detection of mumps virus RNA in clinical specimens. J Clin Microbiol. 45(9): 2902-2908, 2007. PubMed: 17652480
Xu P, et al. Rescue of wild-type mumps virus from a strain associated with recent outbreaks helps to define the role of the SH ORF in the pathogenesis of mumps virus. Virology 417(1): 126-136, 2011. PubMed: 21676427
Nucleotide (GenBank) : JN012242.1 Mumps virus strain MuV-IA, complete genome
Nucleotide (GenBank) : DQ661745.1 Mumps virus strain MUM/Iowa.US/2006 small hydrophobic protein mRNA, complete cds
