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Mumps rubulavirus
Mumps rubulavirus
規格:
貨期:
編號:B235315
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Mumps rubulavirus
商品貨號
B235315
Classification
Paramyxoviridae, Rubulavirus
Deposited As
Mumps
Agent
Mumps rubulavirus
Strain
Enders
Common Name
Mumps virus
Applications
This strain was used for preparation of skin test and viral V and S complement fixation antigens.
Respiratory research
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Comments
This strain was used for preparation of skin test and viral V and S CF antigens. It is of low virulence for human volunteers. GenBank: GU980052.1
Effect on Host
CPE, cell rounding, syncytia formation, and sloughing
Recommended Host
SL-29 (ATCC® CRL-1590™)
Growth Conditions
Temperature: 36°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 36°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 5-11 days
Name of Depositor
JF Enders, Children's Medical Center, Boston, Massachusetts
Source
Pooled saliva from patients, Massachusetts
References

Andrews CH. A short description of the Myxovirus group (Influenza and related viruses). Virology. 2: 176-184, 1955. PubMed: 13267985.

Enders JF, et al. Immunity in mumps: I. Experiments with monkeys (Macacus mulatta). The development of complement-fixing antibody following infection and experiments on immunization by means of inactivated virus and convalescent human serum. J. Exp. Med. 81: 93-117, 1945. PubMed: 19871448.

Henle G, et al. The reactivity of various human sera with mumps complement fixation antigens. J. Exp. Med. 1: 133-147, 1948. PubMed: 18871883.

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