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當前位置: 首頁 > ATCC代理 > Mumps rubulavirus B163025
Mumps rubulavirus
Mumps rubulavirus
規格:
貨期:
編號:B163025
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Mumps rubulavirus
商品貨號
B163025
Classification
Paramyxoviridae, Rubulavirus
Deposited As
Mumps
Agent
Mumps rubulavirus
Strain
Enders
Common Name
Mumps virus
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen
Storage Conditions
-70°C or colder
Comments
Same strain as VR-106. Adapted at CDC to grow in continuous cell line NCI-H292 (HuT-292)--see Castells et al. Arch Virol. 115:277-288, 1990.
After adsorbing virus, feed with medium containing trypsin but NO serum.
Effect on Host
Cytopathic effects in tissue culture. No CPE or extremely subtle CPE, cells thicken and some clumping. Hemadsorption and hemagglutination with chicken or guinea pig red blood cells.
Recommended Host
NCI-H292 (ATCC® CRL-1848™)
Growth Conditions
Temperature: 36°C
Atmosphere: 5% CO2 in air recommended
Incubation: 6 to 12 days at 36°C rocking, a 5% CO2 in air atmosphere is recommended.
Recommendations for Infection: For best results cells should be 48 to 72 hours old and 100% confluent.
Name of Depositor
JC Hierholzer
Source
Pooled saliva from patients, Massachusetts
References

Enders JF, et al. Immunity in mumps: I. Experiments with monkeys (Macacus mulatta). The development of complement-fixing antibody following infection and experiments on immunization by means of inactivated virus and convalescent human serum. J. Exp. Med. 81: 93-117, 1945. PubMed: 19871448.

Levens JH, Enders JF. . Science 102: 117, 1945.

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