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Vaccinia virus
Vaccinia virus
規格:
貨期:
編號:B163058
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Vaccinia virus
商品貨號
B163058
Classification
Poxviridae, Orthopoxvirus
Agent
Vaccinia virus
Strain
CL
Applications
This strain was used by J. Craigie in studies of antigenic composition and by T. M. Rivers and collaborators in studies of chemical composition of vaccinia virus.
It is a dermal strain and produces rather small, opaque, white pocks on chorioallantoic membranes.
Biosafety Level
2 Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (Laboratory safety is discussed in the current publication of the Biosafety in Microbiological and Biomedical Laboratories, from the U.S. Department of Health and Human Services Centers for Disease Control and Prevention and National Institutes for Health)

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Probably derived from New York City Department of Health strain of vaccinia
Product Format
frozen
Storage Conditions
?-70°C or colder
Comments
REFERENCE STRAIN
This strain was used by J. Craigie in studies of antigenic composition and by T. M. Rivers and collaborators in studies of chemical composition of vaccinia virus.
It is a dermal strain and produces rather small, opaque, white pocks on chorioallantoic membranes.
It is non-pathogenic for mice (i. c.).
The virus is very resistant and may contaminate the laboratory.
Effect on Host
Cell culture: CPE is refractile cell rounding.
CPE is refractile cell rounding in culture.
In vivo: Causes pocks on chorioallantoic membrane.
Recommended Host
Production Host: LLC-MK2 Derivative cells (ATCC CCL-7.1)
Alternate Host: Chicken embryo chorioallantoic membrane (CAM)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plant cells 24-48 hours in advance and infect when cultures are 90-95% confluent. Remove medium and inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted to provide a MOI of about 0.1. Adsorb 30 minutes at 37°C in a humidified 5% CO2 atmosphere, rocking every 7-10 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 1-3 days at 37°C in a humidified 5% CO2 atmosphere.
Name of Depositor
F. Fenner; Australian National University
Chain of Custody
ATCC <-- F Fenner
Source
Probably derived from New York City Department of Health strain of vaccinia
References

Fenner, F and Burnet, FM. A short description of the Poxvirus group (Vaccinia and related viruses). Virology 4: 305-314, 1957. PubMed: 13496547.

Craigie J. . Br. J. Exp. Pathol. 13: 259, 1932.

Parker RF, et al. Further studies of the infectious unit of Vaccinia. J. Exp. Med. 74: 263-281, 1941.

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