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Hepatitis A virus
Hepatitis A virus
規格:
貨期:
編號:B163204
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Hepatitis A virus
商品貨號
B163204
Classification
Picornaviridae, Hepatovirus
Deposited As
Hepatitis A
Agent
Hepatitis A virus
Strain
HAS-15
Applications
Enteric Research
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen
Storage Conditions
-70°C or colder
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Images
ATCC VR-2281 micrograph images
Comments
Genome contains 18-nucleotide deletion between VPI nucleotides 3383-2301. Attenuated for chimpanzees at 200 ng dose.
Effect on Host
Gradual deterioration of cells; titer by ELISA or by RT-PCR (cells become persistently infected). Subtle CPE may be missed. Monolayer does not fill in, cells round and deteriorate, but remain attached indicating persistent infection.
Recommended Host
FRhK-4 (ATCC® CRL-1688™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 70-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 14-21 days at 37°C in a humidified 5% CO2 atmosphere.
Name of Depositor
BH Robertson
U.S. Patent
U.S. Patent Number
Source
Human feces
References

Robertson BH, et al. Large scale production of hepatitis A virus in cell culture: effect of type of infection on virus yield and cell integrity. J. Gen. Virol. 69: 2129-2134, 1988. PubMed: 2841419

Cross References

Nucleotide (GenBank) : JQ425480.1 Hepatitis A virus strain HAS-15, complete genome

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