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Vaccinia virus ts mutant
Vaccinia virus ts mutant
規格:
貨期:
編號:B163326
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Vaccinia virus ts mutant
商品貨號
B163326
Classification
Poxviridae, Orthopoxvirus
Deposited As
Vaccinia virus, Orthopoxvirus
Agent
Vaccinia virus ts mutant
Strain
IHD-W Dts22
Applications
The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location G2, a positive transcription elongation factor.
Biosafety Level
2 BSL 2Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses.
Isolation
This conditional lethal mutant, originally designated plaque isolate no. 3145, was isolated from the IHD-W strain of Vaccinia by S. Dales. The IHD-W strain was derived from an IHD-J strain of Vaccinia
Product Format
frozen
Storage Conditions
-70°C or colder
Comments
Not described in the original publication, but presumed to have been selected after mutagenesis with nitrosoguanidine. The non-permissive incubation temperature is 39.5 C. This mutant was not assigned to a Dales EM category. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location G2, a positive transcription elongation factor.
Effect on Host
Cytopathic effects (CPE): enlargement, plaques with rounding and eventual sloughing
CPE: enlargement, plaques with rounding and eventual sloughing
Growth Medium
ATCC® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
Recommended Host
Production Host: BSC-40 (ATCC CRL-2761)

Recommended Host: BSC-40 (ATCC CRL-2761)

Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Growth Conditions
1-3 days at 31 C with 5% CO2
Name of Depositor
S Dales & R Condit
References

Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807

Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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