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143B
143B
規格:
價格:
編號:B163573
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
143B
商品貨號
B163573
Organism
Homo sapiens, human
Tissue
bone
培養基
Product Format
frozen
Morphology
mixed
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
osteosarcoma
Age
13 year old
Gender
female
Ethnicity
Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Clinical Data
13 year old
Caucasian
female
Comments
Thymidine kinase negative (TK-).
This is a human osteosarcoma cell line.
Complete Growth Medium
Minimum essential medium (Eagle) in Earle's BSS with 0.015 mg/ml 5-bromo-2'-deoxyuridine, 90%; fetal bovine serum, 10%
Subculturing
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution and allow the cells to sit at room temperature (or at 37°C) until they detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Medium Renewal: 2 to 3 times per week
Cryopreservation
90% FBS; 10% DMSO
Culture Conditions
Temperature: 37°C

STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18
Name of Depositor
Wistar Institute
Deposited As
Homo sapiens
U.S. Patent Number
References

Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 8709256

Roller RJ, et al. Structure and function in the herpes simplex virus 1 RNA-binding protein US11: mapping of the domain required for ribosomal and nucleolar association and RNA binding in vitro. J. Virol. 70: 2842-2851, 1996. PubMed: 8627758

Hofhaus G, et al. Respiration and growth defects in transmitochondrial cell lines carrying the 11778 mutation associated with Leber's hereditary optic neuropathy. J. Biol. Chem. 271: 13155-13161, 1996. PubMed: 8662757

Hocking AM, et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996. PubMed: 8702651

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