Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
osteosarcoma
Age
13 year old
Gender
female
Ethnicity
Caucasian
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
Clinical Data
female
Caucasian
13 year old
Genes Expressed
genome, partial [tk]
Comments
Contains a thymidine kinase (TK+) plasmid (PMBKTK).
Complete Growth Medium
Minimum essential medium (Eagle) in Earle's BSS with HAT (0.1 mM hypoxanthine, 400 nM aminopterin, 0.16 mM thymidine), 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution and allow the cells to sit at room temperature (or at 37C) until they detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
