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SVG p12

規格:

價格:2500.00

編號:B165770

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

SVG p12

商品貨號

B165770

Organism

Homo sapiens, human

Tissue

brain

培養基

Cell Type

astroglia; SV40 transformed

Product Format

frozen

Morphology

fibroblast

Culture Properties

adherent

Biosafety Level

2 [Cells contain polyomavirus DNA sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age

fetus, first trimester

Applications

The are susceptible to infection by JC virus, and may be useful in, detecting and cultivating other human neurotropic viruses.

Storage Conditions

liquid nirogen vapor phase

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

The SVG p12 cell line was established by transfecting cultured human fetal glial cells from brain material dissected from 8 to 12 week old embryos with DNA from an ori - mutant of SV40.

Genes Expressed

SV40 T protein; glial fibrillary acidic protein (GFAP)

Cellular Products

SV40 T protein; glial fibrillary acidic protein (GFAP)

Virus Susceptibility

JC polyomavirus

Comments

The cells express SV40 T antigen.

These cells were recently found to contain infectious BK polyomavirus (BKPyV) of UT strain and a spectrum of defective mutants of this virus.

Ref

Henriksen, S et al. The human fetal glial cell line SVG p12 contains infectious BK polyomavirus (BKPyV). J. Virol. 2014 Apr 23. [Epub ahead of print] PubMed: 24760884

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels. Seed new flasks (75 cm²) with 5 X 10⁵ cells.
Incubate cultures at 37°C.

Medium Renewal: 2 to 3 times per week

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nirogen vapor phase

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: X,Y
CSF1PO: 10,11,12
D13S217: 8,13,14
D16S5396: 10,11,12
D5S818: 11,12,14
D7S820: 10,12,13
TH01: 6,7,9.3
TPOX: 8,10,11
vWA: 15,16,18,19

Name of Depositor

The United States of America

U.S. Patent Number

4,707,448

References

Major EO. Immortal line of human fetal glial cells. US Patent 4,707,448 dated Nov 17 1987

Henriksen, S et al. The human fetal glial cell line SVG p12 contains infectious BK polyomavirus (BKPyV). J. Virol. 2014 Apr 23. [Epub ahead of print] PubMed: 24760884

B242958：pRSVgpt

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