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An upper limit for population doublings has not been established for primary fibroblasts when cultured with Fibroblast Basal Medium supplemented with Fibroblast Growth Kit, Low serum (2% FBS).
The final concentration for each component is as follows:

• L-glutamine: 7.5 mM
• rh FGF basic: 5 ng/mL
• rh Insulin: 5 µg/mL
• Hydrocortisone: 1 µg/mL
• Ascorbic acid: 50 µg/mL
• Fetal bovine serum: 2%

1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
2. Thaw the components of the growth kit just prior to adding to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath, and shake to dissolve any precipitates, prior to adding to the basal medium.
3. Obtain one bottle of Fibroblast Basal Medium (480 mL) from cold storage.
4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the volume of each growth kit component, as indicated in Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer.

Table 1. If using the Fibroblast Growth Kit–Serum-Free (ATCC^® PCS-201-040), add the indicated volume for each component in the order shown.

Component Volume Volume Sterility Tests Final Concentration Functional Tests L-glutamine C of A 18.75 mL 2026-06-16 明舟生物訂購流程 2026-08-18 開學季實驗福利｜限時滿贈耗材自選禮包，階梯加碼最高贈千元！ 2026-05-29 明舟生物 618 菌株狂歡節｜三重福利送不停！ 2019-06-29 ATCC細胞運輸和處理 2019-06-28 其他海外品牌代理 2019-05-23 ATCC代理 2019-05-22 購買須知 B235670：Primary Bladder Fibroblast Cells; Normal, Human B230676：Primary Uterine Fibroblast Cells; Normal, Human (HUF) B227285：Primary Dermal Fibroblast Normal; Human, Neonatal (HDFn) B213506：Fibroblast Growth Kit-Serum-free B192575：Fibroblast Basal Medium 王經理 手機號：17280875617 QQ：1438578920 胡經理 手機號：13345964880 QQ：2438244627 周經理 手機號：17757487661 QQ：1296385441 于經理 手機號：18067160830 QQ：2088210172 沈經理 手機號：19548299266 QQ：2662369050 李經理 手機號：13626845108 QQ：972239479 友情鏈接 中國林業微生物菌種保藏管理中心 中國工業微生物菌種保藏管理中心 中國普通微生物菌種保藏管理中心 美國國家生物技術信息中心(NCBI) SIB Bioinformatics Resource Portal https://www.weibo.com/p/10050 中國典型培養物保藏中心 ATCC菌株、細胞 菌株，慢病毒，細菌，真菌，質粒載體 我的帳戶 登錄 注冊 購物車 我的訂單 用戶中心 客戶支持 在線客服 業務QQ 免費電話 在線支付 留言反饋 聯系我們 業務咨詢:0574-87157013 郵箱:mingzhoubio@163.com 浙江省寧波市高新區晶輝路866弄33號智造港E4幢3樓 Copyright ?2019 寧波明舟生物科技有限公司 mingzhoubio.com All Rights Reserved. 浙ICP備19018319號-1