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Fibroblast Growth Kit-Serum-free
Fibroblast Growth Kit-Serum-free
規(guī)格:
價(jià)格:
編號(hào):B213506
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Fibroblast Growth Kit-Serum-free
商品貨號(hào)
B213506
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen 1 kit
培養(yǎng)基
Storage Conditions
-20° (or -70°C for long-term storage)
Comments

The kit contains components that when added to the fibroblst basal medium ATCC PCS-201-030 create a complete serum-free culture environment for fibroblasts. The final concentration for each component is as follows:

  • HLL Supplement: HSA 500 µg/mL, linoleic acid 0.6 mM, lecithin 0.6 µg/mL
  • L-Glutamine: 7.5 mM
  • rh FGF basic: 5 ng/mL
  • rh EGF/TGF-1 Supplements: 5 ng/mL and 30 pg/mL, respectively
  • rh Insulin: 5 µg/mL
  • Hydrocortisone: 1 µg/mL
  • Ascorbic acid: 50 µg/mL
Complete Growth Medium
  1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
  2. Thaw the components of the growth kit just prior to adding to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath, and shake to dissolve any precipitates, prior to adding to the basal medium.
  3. Obtain one bottle of Fibroblast Basal Medium (480 mL) from cold storage.
  4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
  5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the volume of each growth kit component, as indicated in Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer.

    6. Table 1. If using the Fibroblast Growth Kit–Serum-Free (ATCC® PCS-201-040), add the indicated volume for each component in the order shown.

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      Component
                                              
                                    
                                                                    
                                                                                                                                                                                                
      
                                    
                                    
      
                                        
      
                                            Volume                                        
      
                                        
      
                                            
      Volume
                                              
      
                                    
      
                                                                                                                                                                    
                                    
      
                                        
      
                                            Sterility Tests                                        
      
                                        
      
                                            
      Final Concentration
                                              
      
                                    
      
                                                                    
      
                                                                                                                                                                                                
      
                                    
                                    
      
                                        
      
                                            Functional Tests                                        
      
                                        
      
                                            
      L-glutamine
                                              
      
                                    
      
                                                                                                                                                                    
                                    
      
                                        
      
                                            C of A                                        
      
                                        
      
                                            
      18.75 mL
                                              
      
                                    
      
                                                                    
      
                                                                                                                                                            
                            
                        
                        
                        
                        
      
                            

      

      
	
        
			
      

      
			

                        
      
                        
      
                            
      
                                
        
                                    
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