Restriction digests of the clone give the following sizes (kb): BamHI--7.4; EcoRI--7.4; SacI/XbaI--3.2, 1.7, 1.5, 1.0.
The wild type ADH promoter is active when cells are grown in glucose media but can be repressed 2-10 fold on non-fermentable carbon sources.
High copy number shuttle expression vector.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504
