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p424 TEF

規(guī)格:

價(jià)格:

編號(hào):B207789

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

p424 TEF

商品貨號(hào)

B207789

Designations

p424 TEF

Depositors

M Funk

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 6.2490000724792480

Vector: p424 TEF (plasmid)

Promoters: Promoter for expression TEF

Construction: pRS424

Marker(s):TRP1,ampR

Construct size (kb): 6.249000072479248

Features: marker(s): TRP1
marker(s): ampR
promoter for expression: TEF
replicon: 2 micron
MCS: XhoI...XbaI
terminator: CYC1

Applications

YE-type (episomal) shuttle vector

YX-type (expression) shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--6.2; EcoRI--6.2; SacI/XbaI--3.2, 1.7, 1.0, 0.44.

High copy number shuttle expression vector.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504