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當前位置: 首頁 > ATCC代理 > pG7/T1 [N5-7/U7-T1] B177441
pG7/T1 [N5-7/U7-T1]
pG7/T1 [N5-7/U7-T1]
規格:
價格:詢價
編號:B177441
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pG7/T1 [N5-7/U7-T1]
商品貨號
B177441
Designations
pG7/T1 [N5-7/U7-T1]
GenBank Number

J02636

培養基
Species
Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors
A Tzagoloff
Vector
Construct size (kb): 14.39999961853027
Insert
DNA: genomic
Insert lengths(kb): 6.699999809265137
Gene product: coenzyme QH2-cytochrome c reductase [COR1]
Insert Size (kb)
6.700
Genotype
MATalpha ura3-1 cor1-1
Mating Type
alpha
Media
ATCC® Medium 1688: Glycerol enriched medium
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The insert contains the following sites separated by (kb) (approximate): EcoRI- 1.0- EcoRI- 0.1- SphI- 0.3- EcoRI- 1.0- XhoII- 1.6- EcoRI- 0.3- XbaI- 1.1- XhoI.
Carries plasmid pG7/T1 with wild-type gene
The smallest subclone capable of complementing a cor1-1 mutation was a 3.0 kb XhoII fragment. This region contains a continuous 1.368 kb open reading frame encoding a protein of 456 amino acids with a molecular weight of 50 kDa.
The probe, pG7/T1, has been deposited in Escherichia coli RR1 as ATCC 37665 and in Saccharomyces cerevisiae N5-7/U7 as ATCC 66316.
Classification
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Tzagoloff A, et al. Assembly of the mitochondrial membrane system. Characterization of COR1, the structural gene for the 44-kilodalton core protein of yeast coenzyme QH2-cytochrome c reductase. J. Biol. Chem. 261: 17163-17169, 1986. PubMed: 3023384

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