The insert contains the following restriction sites (kb):
BamHI--4.549, 3.417; EcoRI--5.215, 2.686; KpnI--6.365, 1.591;
SalI--7.956; SfiI--7.956; XhoI--uncut Description of cloned agent: Name: SV40 Classification: Polyomavirus Plasmid copy#: High-copy number (pUC19 has a ColEI ori) Viable virus clone. Verified by lipofection of EcoRI-linearized plasmid DNA into ATCC CV-1 cells (passage# 40) by J. Lednicky (lipofection date: 12 Oct. 2000). The viral regulatory region of this construct is complex (non-archetypal) but distinct from the complex regulatory region of SV40-777-5. Plasmid size: 7.956 kb [2.686 kb (pUC19
vector) + 5.270 kb (SV40-777-5 insert)] |
