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phigf1
phigf1
規格:
價格:
編號:B181067
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
phigf1
商品貨號
B181067
Designations
phigf1
Species
Homo sapiens, human
培養基
Depositors
GI Bell
Vector
Construct size (kb): 4.5
Insert
DNA: cDNA
Insert lengths(kb): 0.659
Tissue: liver
Gene product: insulin-like growth factor 1 [IGF1]
Alleles: A1, A2
Insert Size (kb)
0.659
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
The insert contains the following restriction sites (nt from the 5' end): RsaI--323; BamHI--420.
Restriction digests of the clone give the following sizes (kb): HindIII--4.6; EcoRI--4.6; AvaI--4.6; BamHI--3.5, 1.05; PstI--3.9, 0.68.
The insert extends from the second nucleotide of the codon for amino acid -15 of the signal peptide to the poly(A) tract.
Detects genomic EcoRI fragments of 8.0, 6.6, and 4.3 kb in human cell lines, and fragments of 6.7, 2.1, and 1.9 kb in mouse.
This probe also reveals a HindIII polymorphism which appears to be linked to the PvuII polymorphism.
The vector pKT218 is pBR322 with an approximate 600 bp deletion of the ampR gene. The deletion starts near the beginning of the amp gene but is distal to the amp promoter and initiation ATG site and ends at the PstI site. The sequence from the ATG start of the amp gene to the PstI site is : ATG AGT ATT CAA GCT GCA G
References

Bell GI, et al. Sequence of a cDNA clone encoding human preproinsulin-like growth factor II. Nature 310: 775-777, 1984. PubMed: 6382021

Tricoli JV, et al. Localization of insulin-like growth factor genes to human chromosomes 11 and 12. Nature 310: 784-786, 1984. PubMed: 6382024

Morton CC, et al. Human genes for insulin-like growth factors I and II and epidermal growth factor are located on 12q22----q24.1, 11p15, and 4q25----q27, respectively. Cytogenet. Cell Genet. 41: 245-249, 1986. PubMed: 3486749

Graeme I Bell, personal communication

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