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phigf1
phigf1
規(guī)格:
價格:
編號:B214943
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
phigf1
商品貨號
B214943
Designations
phigf1
Species
Homo sapiens, human
培養(yǎng)基
Vector
Construct size (kb): 4.5
Insert
DNA: cDNA
Insert lengths(kb): 0.659
Tissue: liver
Gene product: insulin-like growth factor 1 [IGF1]
Alleles: A1, A2
Insert Size (kb)
0.659
Related Products
59944
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The insert contains the following restriction sites (nt from the 5' end): RsaI--323; BamHI--420.
Restriction digests of the clone give the following sizes (kb): HindIII--4.6; EcoRI--4.6; AvaI--4.6; BamHI--3.5, 1.05; PstI--3.9, 0.68.
The insert extends from the second nucleotide of the codon for amino acid -15 of the signal peptide to the poly(A) tract.
Detects genomic EcoRI fragments of 8.0, 6.6, and 4.3 kb in human cell lines, and fragments of 6.7, 2.1, and 1.9 kb in mouse.
This probe also reveals a HindIII polymorphism which appears to be linked to the PvuII polymorphism.
The vector pKT218 is pBR322 with an approximate 600 bp deletion of the ampR gene. The deletion starts near the beginning of the amp gene but is distal to the amp promoter and initiation ATG site and ends at the PstI site. The sequence from the ATG start of the amp gene to the PstI site is : ATG AGT ATT CAA GCT GCA G
This construct comes as 200 ng of purified plasmid DNA. It is also available already in E. coli as ATCC# 59944.
References

Bell GI, et al. Sequence of a cDNA clone encoding human preproinsulin-like growth factor II. Nature 310: 775-777, 1984. PubMed: 6382021

Tricoli JV, et al. Localization of insulin-like growth factor genes to human chromosomes 11 and 12. Nature 310: 784-786, 1984. PubMed: 6382024

Morton CC, et al. Human genes for insulin-like growth factors I and II and epidermal growth factor are located on 12q22----q24.1, 11p15, and 4q25----q27, respectively. Cytogenet. Cell Genet. 41: 245-249, 1986. PubMed: 3486749

Graeme I Bell, personal communication

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