Note that activities with high potential for aerosol production require BSL 3 facilities and practices.
For a time, prior to the acceptance of the C. pneumoniae strains as a separate species, the strains were refered to as TWAR, after the isolates TW-183 and AR-39. AR-39 is available as ATCC 53592.
The inclusions are iodine stain negative (contain no glycogen).
Suggested protocol for propagation: Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour.
Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 µg/mL cycloheximide. Incubate at 37°C for 72 hours.
Infection of TC is enhanced by subconfluent monolayer of cells.
This preparation has tested positive for Mycoplasma contamination.
The inclusions are iodine stain negative (contain no glycogen). The TWAR strains (now C. pneumoniae) of Chlamydia are named after TW183 and AR39. |
