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<em>Chlamydophila pneumoniae</em>

規格:

貨期:

編號:B227156

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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產品名稱

Chlamydophila pneumoniae

商品貨號

B227156

Classification

Chlamydiaceae, Chlamydophila

Deposited As

Chlamydia pneumoniae Grayston et al.

Agent

Chlamydophila pneumoniae

Strain

CM-1

Applications

Respiratory research

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format

frozen 1 mL per vial

Storage Conditions

-70°C or colder

Comments

This isolate was identified as C. pneumoniae by culture characteristics, morphology of inclusion bodies, and reaction with monoclonal antibodies by depositor. Item isolated on cycloheximide-treated HeLa 229 cells, and found to be serologically identical to the reference strain TW183 by micro-immunofluorescent test.

Effect on Host

Intracellular glycogen negative inclusion bodies visualized by fluorescent staining

Recommended Host

HEp-2 (ATCC^® CCL-23™)

Growth Conditions

Temperature: 36°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80-95% confluent. Vortex material with 1mm glass beads to disrupt cells. Remove cell growth medium and inoculate with disrupted material. Centrifuge at 1500 x g at 25°C for 1 hour. End adsorption by adding agent growth medium.
Incubation: 3 days

Name of Depositor

KH Wong, Centers for Disease Control and Prevention

Special Collection

NCRR Contract

Source

Sputum of pneumonia patient, Georgia

References

Black CM, et al. Antigenic variation among strains of Chlamydia pneumoniae. J. Clin. Microbiol. 29: 1312-1316, 1991. PubMed: 1885727

Cross References

Nucleotide (GenBank) : U44033 Chlamydia pneumoniae putative ribonuclease P RNA gene.

B192482：Chlamydophila pneumoniae

B181539：Chlamydophila pneumoniae

B166164：Chlamydophila pneumoniae

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