Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Storage Conditions
-70°C or colder
Comments
This isolate was deposited to ATCC after five passages in weanling mice and two passages in suckling mice. It was adapted to growth in cell culture by three passages in Vero cells (ATCC® CCL-81™).
Effect on Host
CPE in the form or refractile rounding and sloughing
Recommended Host
Vero cells (ATCC® CCL-81™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plant cells 24 hours in advance and infect when cultures are 80-90% confluent. Remove medium and rinse cell sheet with 1X DPBS. Remove 1X DPBS and then inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted to provide a MOI of about 0.01. Adsorb for 1 hour at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 1 to 4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE are well advance through 90% of the culture.
Name of Depositor
SE Grossberg, Medical College of Wisconsin
References
Helwig FC and Schmidt CH. A filter-passing agent producing interstitial myocarditis in anthropoid apes and small animals. Science 102:31-33, 1945. PubMed 17767415
