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Encephalomyocarditis virus
Encephalomyocarditis virus
規(guī)格:
貨期:
編號(hào):B208468
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Encephalomyocarditis virus
商品貨號(hào)
B208468
Classification
Picornaviridae, Cardiovirus
Agent
Encephalomyocarditis virus
Strain
MM
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Images
VR-1763
Comments
This item was deposited at ATCC® after six passages in weanling mice and three passages in suckling mice. A seed stock was produced by a single passage in BHK-21 cells.
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
BHK-21 (ATCC® CCL-10™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-95% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-3 days
Name of Depositor
SE Grossberg, Medical College of Wisconsin
Source
Isolated from dead mouse found in the basement of a patient with poliomyelitis, New York, 1942
References

Helwig FC, Schmidt EC. A filter-passing agent producing interstitial myocarditis in anthropoid apes and small animals. Science 102: 31-33, 1945.

Oberste MS, et al. Human febrile illness caused by encephalomyocarditis virus infection, Peru. Emerg. Infect. Dis. 15: 640-646, 2009. PubMed: 19331761

Pindak FF, Schmidt JP. Propagation of MM virus in continuous cell lines. Appl. Microbiol. 17: 815-818, 1969. PubMed: 4307881

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