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Preceptrol®

no

Genotype

F- thi-1 endA1 supE44 hsdR17 proC::Tn10 lacI+ delta(lacZ)M15 pro- lambda-

Type Strain

no

Comments

It is resistant to tetracycline at 10-20 ug/ml.

derived from MM294 and isolated by D. Gelfand

A host strain providing efficient transformation and alkaline SDS screening of plasmid recombinants. It has a lac alpha acceptor for blue/white colony discrimination and does not completely shut off lacZ on multicopy plasmids.

Medium

ATCC^® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline

Growth Conditions

Temperature: 37.0°C

Name of Depositor

JC Hunter-Cevera

References

Jennie C Hunter-Cevera, personal communication