| Vector |
Construct size (kb): 3.859999895095825 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: SacI BstXI SacII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GPX4
Gene name: glutathione peroxidase 4 (phospholipid hydroperoxidase)
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19
Tissue: testis
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 0.896
Cross references: DNA Seq. Acc.: X71973
Nucleotides 1-896 of the insert correspond to
nucleotides 1-896 of X71973. Insert lengths(kb): 0.8960000276565552 Tissue: testis Gene product: glutathione peroxidase 4 (phospholipid hydroperoxidase) [GPX4] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.9; BamHI--3.3, 0.6; BglII--3.9; SalI--3.5, 0.4; SmaI--3.1, 0.8. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.37; BglI--0.82; BglII--0.48; BspHI--0.74; HincII--0.24, 0.33; NcoI--0.63, 0.81; SalI--0.33; SmaI--0.17; XhoII--0.37, 0.48. |
