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pBSGPX4
pBSGPX4
規格:
價格:
編號:B206787
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pBSGPX4
商品貨號
B206787
Designations
pBSGPX4
GenBank Number

X71973

培養基
Species
Homo sapiens, human
Depositors
F Chu
Vector
Construct size (kb): 3.859999895095825
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: SacI BstXI SacII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GPX4
Gene name: glutathione peroxidase 4 (phospholipid hydroperoxidase)
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19
Tissue: testis
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 0.896
Cross references: DNA Seq. Acc.: X71973
Nucleotides 1-896 of the insert correspond to
nucleotides 1-896 of X71973.
Insert lengths(kb): 0.8960000276565552
Tissue: testis
Gene product: glutathione peroxidase 4 (phospholipid hydroperoxidase) [GPX4]
Insert Size (kb)
0.896
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.9; BamHI--3.3, 0.6; BglII--3.9; SalI--3.5, 0.4; SmaI--3.1, 0.8.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.37; BglI--0.82; BglII--0.48; BspHI--0.74; HincII--0.24, 0.33; NcoI--0.63, 0.81; SalI--0.33; SmaI--0.17; XhoII--0.37, 0.48.
References

Esworthy RS, et al. Cloning and sequencing of the cDNA encoding a human testis phospholipid hydroperoxide glutathione peroxidase. Gene 144: 317-318, 1994. PubMed: 8039723

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