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pIB1
pIB1
規(guī)格:
價格:詢價
編號:B183214
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱
pIB1
商品貨號
B183214
Designations
pIB1
Species
Homo sapiens, human
培養(yǎng)基
Depositors
Genentech, Inc., WH Dreger, Genentech, Inc.
Applications
produces protein insulin
Vector
Construct size (kb): 10.60000038146973
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: EcoRI
Vector end: BamHI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
Insert
DNA: Synthetic
DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: INS
Genomic copy number: unique
Gene name: insulin
Contains complete coding sequence?: U
Chromosome: 11; Localization: 11 p15.5
Type of DNA: synthetic
Insert end: EcoRI
Insert end: BamHI
Insert size (kb): 0.1
Cross references:
Insert lengths(kb): 0.1000000014901161
Gene product: insulin [INS]
Alleles: K2, A3, B2, A1, A1, A4, A3, A3, A5, A6, B1, K1, A1, A2, A2, A2
Target Gene: insulin
Insert Size (kb)
0.100
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests on an agarose gel give the following sizes (kb): EcoRI--6.4, 4.1; BamHI--10.5.
The sequence of the insert is AATTCATGTT CGTCAATCAG CACCTTTGTG GTTCTCACCT CGTTGAAGCT TTGTACCTTG TTTGCGGTGA ACGTGGTTTC TTCTACACTC CTAAGACTTA ATAGGATC, including a HindIII site at position 46.
This construct directs the synthesis of a beta-galactosidase-insulin B chain fusion protein regulated by the lac promoter. The B peptide can be released by cyanogen bromide cleavage and used to reconstitute insulin with the product of pIA1, ATCC 31448.
This clone was constructed from pBR322, an EcoRI fragment from lambda plac containing most of the beta-galactosidase gene, and synthetic DNA encoding human insulin B chain.
References

Riggs AD. Method for microbial polypeptide expression. US Patent 4,366,246 dated Dec 28 1982

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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