| Insert |
DNA: Synthetic DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: INS
Genomic copy number: unique
Gene name: insulin
Contains complete coding sequence?: U
Chromosome: 11; Localization: 11 p15.5
Type of DNA: synthetic
Insert end: EcoRI
Insert end: BamHI
Insert size (kb): 0.1
Cross references: Insert lengths(kb): 0.1000000014901161 Gene product: insulin [INS] Alleles: K2, A3, B2, A1, A1, A4, A3, A3, A5, A6, B1, K1, A1, A2, A2, A2 Target Gene: insulin |
| Comments |
Restriction digests on an agarose gel give the following sizes (kb): EcoRI--6.4, 4.1; BamHI--10.5. The sequence of the insert is AATTCATGTT CGTCAATCAG CACCTTTGTG GTTCTCACCT CGTTGAAGCT TTGTACCTTG TTTGCGGTGA ACGTGGTTTC TTCTACACTC CTAAGACTTA ATAGGATC, including a HindIII site at position 46. This construct directs the synthesis of a beta-galactosidase-insulin B chain fusion protein regulated by the lac promoter. The B peptide can be released by cyanogen bromide cleavage and used to reconstitute insulin with the product of pIA1, ATCC 31448. This clone was constructed from pBR322, an EcoRI fragment from lambda plac containing most of the beta-galactosidase gene, and synthetic DNA encoding human insulin B chain. |
