| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--14.5, 12, 11, 1.6, 1.5; BamHI--22, 12.8, 6.5, 2.6, 1.7, 1.4, 0.6; SalI--26, 10; EcoRI--22, 16.5, 6.8, 2.9, 2.6, 2.2; XhoI--27, 9. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. MspI RFLP detected with a 6.5 kb BamHI fragment. Of 41 individuals, 30 were heterozygous for one or both RFLPs detected by cosmid 131 (ATCC 59310, 59311, 59312, 59313). Enzyme(s) not detecting polymorphism: TaqI, RsaI, EcoRI, HindIII, PvuII, BamHI, SacI, XbaI, DraI, BstEII. |
