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The insert was the result of a PCR amplification of mRNA from fetal tissue using a forward primer derived from the first exon of human WNT3 outside the coding domain and a set of degenerate reverse primers to the reverse complement of the mouse Wnt-3 gene corresponding to the last 4 amino acids and the stop codon. The product of this reaction was then PCR amplified again using another forward primer outside the protein encoding domain and a degenerate set of reverse primers to a highly conserved stretch FHWCC.
Restriction digests of the clone gave the following results (in kb): NotI - 4; NotI/EcoRI - 3.0, 1.0; HindIII/XhoI - 4.0; SmaI - uncut; NotI/HindIII - 3.0, 1.0; HindIII - 4.0. - ATCC staff
Due to the use of a gene internal 3' primer in the PCR reaction, the depositor did not retrieve the complete protein encoding sequence of human WNT3. Ref
Roelink H, et al. Molecular cloning and chromosomal localization to 17q21 of the human WNT3 gene. Genomics 17: 790-792, 1993.
Roelink H, et al. Molecular cloning and chromosomal localization to 17q21 of the human WNT3 gene. Genomics 17: 790-792, 1993.
