ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI--3.65; BamHI--3.65; EcoRI--3.0, 0.9; HindIII--3.65; SalI--3.65.
MYF3 is the human homolog of MyoD1; showing approximately 90% amino acid sequence homology.
A cDNA clone for human MYOD1 has been shown to detect HindIII fragments of 2.6 kb in the mouse, 1.5 kb in the hamster, and 7.0 kb in humans.
This is not a full-length clone, and encodes the carboxy-terminal portion of the protein and includes the 3' coding sequences. It includes nucleotides 595-1415; coding sequences end at 801.
This clone detects a genomic EcoRI fragment of 7.5 kb and a mRNA of about 1.8 kb.
References
Braun T, et al. A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T1/2 fibroblasts. EMBO J. 8: 701-709, 1989. PubMed: 2721498
Braun T, et al. Differential expression of myogenic determination genes in muscle cells: possible autoactivation by the Myf gene products. EMBO J. 8: 3617-3625, 1989. PubMed: 2583111
Scrable HJ, et al. Rhabdomyosarcoma-associated locus and MYOD1 are syntenic but spearate loci on the short arm of human chromsome 1. Proc. Natl. Acad. Sci. USA 87: 2182-2186, 1990. PubMed: 2315312
