| Vector |
Construct size (kb): 3.910000085830689 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC19 Intact vector size: 2.686 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ', <- MCS: EcoRI...HindIII, -> promoter: lac, <- replicon: pMB1, <- marker(s): ampR, <- Cross references: DNA Seq. Acc.: X02514 |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--3.9; EcoRI--2.7, 1.2; BglI--1.45, 1.35; PstI--3.9; KpnI--3.7, 0.2. The insert contains the following restriction sites (nt from the 5' end): BglI--20, KpnI--117; NcoI--15, 1063; PvuII--81, 278, 464, 804. Contains the complete coding sequence (nt 15-702). |
| References |
Muller B, et al. Cloning and sequencing of the cDNA encoding the human homologue of the murine immunoglobulin-associated protein B29. Eur. J. Immunol. 22: 1621-1625, 1992. PubMed: 1534761
Brigitte Mueller, personal communication
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