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huB29/pUC19
huB29/pUC19
規格:
價格:
編號:B221358
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
huB29/pUC19
商品貨號
B221358
Designations
huB29/pUC19
GenBank Number

M80461

培養基
Species
Homo sapiens, human
Depositors
B Mueller
Vector
Construct size (kb): 3.910000085830689
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC19
Intact vector size: 2.686
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: EcoRI...HindIII, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: X02514
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IGB
Genomic copy number: unique
Gene name: immunoglobulin-associated beta (B29)
Contains complete coding sequence?: Y
Chromosome: 17; Localization: 17 q23
Tissue: tonsil
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.224
Cross references: DNA Seq. Acc.: M80461
Insert lengths(kb): 1.223999977111816
Tissue: tonsil
Gene product: immunoglobulin-associated beta (B29) [IGB]
Insert Size (kb)
1.224
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--3.9; EcoRI--2.7, 1.2; BglI--1.45, 1.35; PstI--3.9; KpnI--3.7, 0.2.
The insert contains the following restriction sites (nt from the 5' end): BglI--20, KpnI--117; NcoI--15, 1063; PvuII--81, 278, 464, 804.
Contains the complete coding sequence (nt 15-702).
References

Muller B, et al. Cloning and sequencing of the cDNA encoding the human homologue of the murine immunoglobulin-associated protein B29. Eur. J. Immunol. 22: 1621-1625, 1992. PubMed: 1534761

Brigitte Mueller, personal communication

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