Comments
Restriction digests of the clone give the following sizes (kb): BamHI--13.0, 10.0, 6.6, 4.8, 4.0, 3.4 (doublet), 0.6; BglII--13.5, 7.0 (doublet), 5.1, 4.2, 3.1, 1.7, 1.6, 1.45; EcoRI--17.0, 9.2, 9.0, 5.9, 2.9, 1.1;
HindIII--17.5, 9.4, 5.8, 3.5, 3.3, 2.95, 0.45; SstI--11.5 (doublet), 4.9, 3.3, 2.95, 2.4 (doublet), 1.25, 1.1, 0.80, 0.62, 0.45, 0.30.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Includes exons 12 - 24, with exon 24 (and thus the 3' untranslated region) being complete. This corresponds to domains II, III and IV of the protein product.
Additional GenBank/EMBL accession numbers: L01971-L01982.
