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cosmid322
cosmid322
規(guī)格:
價(jià)格:
編號(hào):B206798
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
cosmid322
商品貨號(hào)
B206798
Designations
cosmid322
GenBank Number

L01971

L01972

L01973

L01974

L01975

L01976

L01977

L01978

L01979

L01980

L01981

L01982

L01983

培養(yǎng)基
Species
Homo sapiens, human
Depositors
JF Gusella
Vector
Construct size (kb): 52.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pWE15
Intact vector size: 8.200
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI
Polylinker sites: EcoRI NotI BamHI NotI EcoRI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, kanR, G418R
promoter: T3, T7
replicon: pMB1
Cross references: DNA Seq. Acc.: X65279
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SCN4A
Gene name: sodium channel, voltage-gated, type IV, alpha polypeptide
Contains complete coding sequence?: N
Chromosome: 17; Localization: 17 q23.1-q25.3
Tissue: lymphoblast
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 44.0
Cross references: DNA Seq. Acc.: L01971
DNA Seq. Acc.: L01972
DNA Seq. Acc.: L01973
DNA Seq. Acc.: L01974
DNA Seq. Acc.: L01975
DNA Seq. Acc.: L01976
DNA Seq. Acc.: L01977
DNA Seq. Acc.: L01978
DNA Seq. Acc.: L01979
DNA Seq. Acc.: L01980
DNA Seq. Acc.: L01981
DNA Seq. Acc.: L01982
DNA Seq. Acc.: L01983
Insert lengths(kb): 44.0
Tissue: lymphoblast
Gene product: sodium channel, voltage-gated, type IV, alpha polypeptide [SCN4A]
Insert Size (kb)
44.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--13.0, 10.0, 6.6, 4.8, 4.0, 3.4 (doublet), 0.6; BglII--13.5, 7.0 (doublet), 5.1, 4.2, 3.1, 1.7, 1.6, 1.45; EcoRI--17.0, 9.2, 9.0, 5.9, 2.9, 1.1;
HindIII--17.5, 9.4, 5.8, 3.5, 3.3, 2.95, 0.45; SstI--11.5 (doublet), 4.9, 3.3, 2.95, 2.4 (doublet), 1.25, 1.1, 0.80, 0.62, 0.45, 0.30.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Includes exons 12 - 24, with exon 24 (and thus the 3' untranslated region) being complete. This corresponds to domains II, III and IV of the protein product.
Additional GenBank/EMBL accession numbers: L01971-L01982.
References

McClatchey AI, et al. The genomic structure of the human skeletal muscle sodium channel gene. Hum. Mol. Genet. 1: 521-527, 1992. PubMed: 1339144

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