Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
stream, Moscow Mountain, ID, 1965
Product Format
dried
Type Strain
yes
Comments
strain description
biochemical identification
Medium
ATCC® Medium 711: PYB
ATCC® Medium 711: PYB
ATCC® Medium 997: Fresh water ameba medium
Growth Conditions
Max Temperature: 25.0°C
Min Temperature: 20.0°C
Duration: grown with Escherichia coli ATCC 11775
Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
Subcultivation
Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
Name of Depositor
CCAP
Year of Origin
1965
References
Daggett PM, Nerad TA. The biochemical identification of vahlkampfiid amoebae. J. Protozool. 30: 126-128, 1983. PubMed: 6864593
Darbyshire. . Protistologica 12: 375-387, 1976.
Pandey VS. Effect of temperature on development of the free-living stages of Ostertagia ostertagi. J. Parasitol. 58: 1037-1041, 1972. PubMed: 4641869
Read LK, et al. A new strain of Paratetramitus jugosis from Laguna Figueroa, Baja California, Mexico. Biol. Bull. 165: 241-264, 1983.
