Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
human urine, Texas, 1950
Product Format
dried
Type Strain
no
Comments
Circular ribosomal RNA genes
biochemical identification
Monoclonal antibodies for identification
Medium
ATCC® Medium 711: PYB
Growth Conditions
Temperature: 25.0°C
Duration: grown with Escherichia coli ATCC 11775
Protocol: ATCCNO: 30216 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
Subcultivation
Protocol: ATCCNO: 30216 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
Name of Depositor
W Balamuth
Chain of Custody
ATCC <<--W Balamuth<<--L. Norman (CDC) <<--- Dr. Cox, Dept. Pub. Hlth., Austin, TX
Year of Origin
1950
References
Daggett PM, Nerad TA. The biochemical identification of vahlkampfiid amoebae. J. Protozool. 30: 126-128, 1983. PubMed: 6864593
Clark CG, Cross GA. Circular ribosomal RNA genes are a general feature of schizopyrenid amoebae. J. Protozool. 35: 326-329, 1988. PubMed: 2840492
Fulton C. Methods in cell physiology. vol. 4New York: Academic Press; 1970.
