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cDMD 9-14 [63-1, cDMD9-14]
cDMD 9-14 [63-1, cDMD9-14]
規格:
價格:
編號:B192241
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
cDMD 9-14 [63-1, cDMD9-14]
商品貨號
B192241
Designations
cDMD 9-14 [63-1, cDMD9-14]
GenBank Number

M18533

培養基
Species
Homo sapiens, human
Applications
For plasmid amplification, use a lac Iq host.
Vector
Construct size (kb): 9.100000381469727
Insert
DNA: cDNA
Insert lengths(kb): 6.099999904632568
Tissue: fetal muscle
Gene product: dystrophin (muscular dystrophy, Duchenne and Becker types), includes DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272 [DMD]
Alleles: U2, A1, A2, C1, T1, U1, A1, A1, A2, A2, B1, B2, C2, D2, R1, R2, T1, T2, T2, T3, D1
Insert Size (kb)
6.100
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.1, 3.0.
For plasmid amplification, use a lac Iq host.
Inserted into EcoRI site with 5' end closer to T7 promoter. Contains internal HindIII (3), PstI (4), BamHI (2), HincII (3), BglII (2), and XbaI sites.
Codominant inheritance of the MspI RFLP demonstrated in 3 two-generation families.
ATCC 57676 has been converted to meet the requirements of the Budapest Treaty for patent deposits. A compliance form is not required for ATCC 57676, but is for ATCC 57677.
Includes probes 9-14. A BamHI digest gives probes 9, 10, and 11-14. Detects genomic HindIII fragments of 7.8, 1, 8.3, 2.3, 1, 8.8, 6, 3.5, 2.55, 2.8, 6.6, 12, 2.4, 1.45, 1.5, 1.9, 2.1, 5.2, 6.8, 10, 1.8, 5.9, 7.8, and 6 kb.
Of the DMD cDNA clones, 1-2a includes nucleotides 1-1538; 2b-3: 1455 to approximately 2600; 4-5a: 2600-4550; 5b-7: 4400-6900; 8: 6900-7800; 9-14: 7800-13900 (in base pairs from the 5' end of the cDNA).
Enzyme(s) not detecting polymorphism: HindIII.
References

Lindlof M, et al. Gene deletions in X-linked muscular dystrophy. Am. J. Hum. Genet. 44: 496-503, 1989. PubMed: 2929594

Wagner M, et al. MspI RFLP for Duchenne muscular dystrophy cDNA subclone 9. Nucleic Acids Res. 17: 3328, 1989. PubMed: 2471152

Den Dunnen JT, et al. Topography of the Duchenne muscular dystrophy (DMD) gene: FIGE and cDNA analysis of 194 cases reveals 115 deletions and 13 duplications. Am. J. Hum. Genet. 45: 835-847, 1989. PubMed: 2573997

Lucotte G, et al. Molecular deletion patterns in Duchenne muscular dystrophy patients. Ann. Genet. 32: 214-219, 1989. PubMed: 2610487

Liechti-Gallati S, et al. RFLPs for Duchenne muscular dystrophy cDNA clones 9 and 10. Am. J. Hum. Genet. 46: 1090-1094, 1990. PubMed: 1971151

Dominguez-Steglich M, et al. The dystrophin gene is autosomally located on a microchromosome in chicken. Genomics 8: 536-540, 1990. PubMed: 2286374

Koenig M, et al. Complete cloning of the Duchenne muscular dystrophy (DMD) cDNA and preliminary genomic organization of the DMD gene in normal and affected individuals. Cell 50: 509-517, 1987. PubMed: 3607877

Koenig M, et al. The complete sequence of dystrophin predicts a rod-shaped cytoskeletal protein. Cell 53: 219-228, 1988. PubMed: 3282674

Louis M Kunkel, personal communication

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