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當前位置: 首頁 > ATCC代理 > cDMD 5b-7 [47-4, cDMD5b-7] B211245
cDMD 5b-7 [47-4, cDMD5b-7]
cDMD 5b-7 [47-4, cDMD5b-7]
規格:
價格:
編號:B211245
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
cDMD 5b-7 [47-4, cDMD5b-7]
商品貨號
B211245
Designations
cDMD 5b-7 [47-4, cDMD5b-7]
GenBank Number

M18533

培養基
Species
Homo sapiens, human
Depositors
LM Kunkel
Applications
For plasmid amplification, use a lac Iq host.
Vector
Construct size (kb): 5.5
Insert
DNA: cDNA
Insert lengths(kb): 2.5
Tissue: fetal muscle
Gene product: dystrophin (muscular dystrophy, Duchenne and Becker types), includes DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272 [DMD]
Alleles: U2, A1, A2, C1, T1, U1, A1, A1, A2, A2, B1, B2, C2, D2, R1, R2, T1, T2, T2, T3, D1
Insert Size (kb)
2.5
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
For plasmid amplification, use a lac Iq host.
Inserted into EcoRI site with 5' end closer to T7 promoter. Contains internal PstI, XbaI, BglII (2), HincII, and HindIII sites.
ATCC 57672 has been converted to meet the requirements of the Budapest Treaty for patent deposits. A compliance form is not needed for ATCC 57672, but is for ATCC 57673.
Of the DMD cDNA clones, 1-2a includes nucleotides 1-1538; 2b-3: 1455 to approximately 2600; 4-5a: 2600-4550; 5b-7: 4400-6900; 8: 6900-7800; 9-14: 7800-13900 (in base pairs from the 5' end of the cDNA).
Includes probes 5b, 6, and 7. Detects genomic HindIII fragments 18, 0.45, 1.3, 1.8, 1.5, 6, 6.2, 11, 4.2, 0.5, 4.1, 1.5, and 10 kb.
Detects HindIII fragments of 10.0, 7.0, 3.8, 3.7, 3.1, 1.6, and 1.2 kb.
References

Lindlof M, et al. Gene deletions in X-linked muscular dystrophy. Am. J. Hum. Genet. 44: 496-503, 1989. PubMed: 2929594

Den Dunnen JT, et al. Topography of the Duchenne muscular dystrophy (DMD) gene: FIGE and cDNA analysis of 194 cases reveals 115 deletions and 13 duplications. Am. J. Hum. Genet. 45: 835-847, 1989. PubMed: 2573997

Lucotte G, et al. Molecular deletion patterns in Duchenne muscular dystrophy patients. Ann. Genet. 32: 214-219, 1989. PubMed: 2610487

Pillers DA, et al. Deletion mapping of Aland Island eye disease to Xp21 between DXS67 (B24) and Duchenne muscular dystrophy. Am. J. Hum. Genet. 47: 795-801, 1990. PubMed: 2220819

Dominguez-Steglich M, et al. The dystrophin gene is autosomally located on a microchromosome in chicken. Genomics 8: 536-540, 1990. PubMed: 2286374

Koenig M, et al. Complete cloning of the Duchenne muscular dystrophy (DMD) cDNA and preliminary genomic organization of the DMD gene in normal and affected individuals. Cell 50: 509-517, 1987. PubMed: 3607877

Koenig M, et al. The complete sequence of dystrophin predicts a rod-shaped cytoskeletal protein. Cell 53: 219-228, 1988. PubMed: 3282674

Louis M Kunkel, personal communication

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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